Hi,
I really need help with my HIF1alpha western blot. I'm trying to detect HIF1alpha in mouse heart essentially but also in rat neonatal cardiac myocytes. I have used several different conditions: PVDF membranes, nitrocellulose, unboiled samples, boiled ones, antibodies from Santa Cruz and from R&D which are used in the litterature. I treated cells with CoCl2 that is an HIF1a stabilizer. Nothing works for me. Please, help me with any suggestions. Thank you.ss