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Thread: Cell culture

  1. #1 Cell culture 
    Forum Bachelors Degree
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    Hi,

    I'm fairly new to experimental cell biology. When you have cells of interest happily growing in media, how do you then make them adhere to coverslips e.g. in a cell-culture plate? I carried out this procedure on Monday but cannot remember what the heck i did. VERY not good.

    Many thanks,

    Tridimity


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  3. #2  
    New Member
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    super glue works just fine.


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  4. #3 Re: Cell culture 
    Moderator Moderator TheBiologista's Avatar
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    Quote Originally Posted by tridimity
    Hi,

    I'm fairly new to experimental cell biology. When you have cells of interest happily growing in media, how do you then make them adhere to coverslips e.g. in a cell-culture plate? I carried out this procedure on Monday but cannot remember what the heck i did. VERY not good.

    Many thanks,

    Tridimity
    You can spin them down onto glass slides if that's what you're interested in? It's a procedure called a cytospin. What exactly is your goal here?
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  5. #4 Re: Cell culture 
    Forum Cosmic Wizard spuriousmonkey's Avatar
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    Quote Originally Posted by tridimity
    Hi,

    I'm fairly new to experimental cell biology. When you have cells of interest happily growing in media, how do you then make them adhere to coverslips e.g. in a cell-culture plate? I carried out this procedure on Monday but cannot remember what the heck i did. VERY not good.

    Many thanks,

    Tridimity
    just ask someone in the lab.

    that's normal practice.
    "Kill them all and let God sort them out."

    - Arnaud Amalric

    http://spuriousforums.com/index.php
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  6. #5  
    Forum Bachelors Degree
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    Thanks guys,

    I figured it out - turns out all it takes is coverslips in nitric acid, and overnight incubation. (I'll leave you to your superglue, Rainycity :wink: )

    Regards
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