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Thread: Immunology Help

  1. #1 Immunology Help 
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    Explain why the following statements are false.
    1. The amino acid sequence of a protein can be determined from the nucleotide sequence of a genomic clone encoding the protein.
    2. Transgenic mice can be prepared by microinjection of DNA into a somatic-cell nucleus.
    3. Primary lymphoid cultures can be propagated indefinitely and are useful in studies on specific subpopulations of lymphocytes.




    4 Using one or more of the methodologies in the reading, outline an experiment that would show that immunoglobulins contain heterodimers.

    5 Describe the genes involved in apoptosis and their effect on it. How is apoptosis involved in hematopoiesis?

    If you can answer any of these questions in pretty good detail your help would be much appreciated. I need some other input for these questions Thanks!


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  3. #2  
    Forum Senior Kukhri's Avatar
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    What ever happened to asking the cute girl who sits behind you in Professor Johnson's class to be your study buddy? She's got to be more fun than we are.


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  4. #3  
    Forum Cosmic Wizard i_feel_tiredsleepy's Avatar
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    These questions are all rather simple, I'm sure a quick look over your notes would answer them.

    And only two of them have anything to do with immunology.

    As an immunology major I was getting all excited to discuss something in my field. T_T
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  5. #4  
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    Since these are homework questions, I will only answer a few. Do the rest yourself.


    2. Method a.) Inject these cells into the inner cell mass (ICM) of mouse blastocysts..Method b.) Inject the male pronucleus with your DNA.

    These methods involve injecting germline cells, not somatic cells.


    5. This should help you:


    http://www.pubmedcentral.nih.gov/art...?artid=2532733

    http://www.weizmann.ac.il/home/ligiv...ne%202001a.pdf

    Hematopoiesis and apoptosis:


    http://bloodjournal.hematologylibrar.../full/90/1/217

    "Cross-linking of major histocompatibility complex (MHC) class II antigens by anti-HLA-DR monoclonal antibody (MoAb; H81.9; IgG2a) results in inhibition of hematopoiesis in canine and human models. Inhibition of hematopoiesis is associated with apoptosis in a proportion of marrow cells. Since in murine macrophages class II cross-linking triggers nitric oxide (NO) production, and NO is thought to affect regulation of hematopoiesis, we investigated whether NO was involved in our models. In murine J774 monocytes/macrophages, MoAb H81.9 did induce NO."
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  6. #5  
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    Quote Originally Posted by i_feel_tiredsleepy
    These questions are all rather simple, I'm sure a quick look over your notes would answer them.

    And only two of them have anything to do with immunology.

    As an immunology major I was getting all excited to discuss something in my field. T_T
    lol awwee
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  7. #6  
    Forum Cosmic Wizard paralith's Avatar
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    Bodom, this is not a homework answers forum. You need to do your own work to answer these questions. At the very least, do your best to answer them on your own, and then we can tell you what we think.
    /moderator mode


    Thanks to all members for not just giving the answers away.
    Man can will nothing unless he has first understood that he must count on no one but himself; that he is alone, abandoned on earth in the midst of his infinite responsibilities, without help, with no other aim than the one he sets himself, with no other destiny than the one he forges for himself on this earth.
    ~Jean-Paul Sartre
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