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Thread: DNA Degradation @ Room Temp

  1. #1 DNA Degradation @ Room Temp 
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    How long does it take 2.5 ug of DNA to degrade at room temperature?


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  3. #2  
    Forum Masters Degree samcdkey's Avatar
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    Depends on whether it is wet or dry. Air dried DNA from blood can be amplified even after 3 months but in wet cotton buds will degrade rapidly. See Lund and Dissing 2003


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  4. #3  
    Forum Cosmic Wizard spuriousmonkey's Avatar
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    Is it contaminated? is it sterile?

    How sterile?

    MQ or RO? Or buffer?
    "Kill them all and let God sort them out."

    - Arnaud Amalric

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  5. #4  
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    Additionally, degradation is usually through the action of DNAses. These require magnesium. EDTA chelates magnesium.

    DNA stored in buffer containing EDTA is typically stable far longer than that stored without, due to the inability of DNAses to act without free magnesium.
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  6. #5  
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    1. it should be sterile
    2. the DNA was sitting in ethanol, but the ethanol is dry now


    thanks!
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