Hi,
I carried out PCR on a polynucleotide of interest recently and then used SDS-PAGE to analyse its migration. At least that was the aim. What happened in reality is I saw no bands except those for the DNA marker, suggesting that I did not extract enough DNA from the plant leaves I was using? Furthermore, the gel itself looks sort of smeary and way, way paler than normal. It was suggested that I may have used too much agarose, yet I weighed out the mass to the exact specified amount.
Please if anyone can help me and tell me what I may be doing wrong that would be great. I would really like to get a handle on PCR & SDS-PAGE!
A sad Tridimity![]()