Thread: FISH and Paint staining

Hi,

Is the method for staining/looking at fetal nuclei from amniotic fluid is usally FISH?

It wont be paint or anything like that would it?

To me paint and FISH look the same, how can i identify the difference?

Thanks

FISH is really only for looking at specific sequences which may be amplified using a DNA probe...

what exactly are you looking for? If its just to show the presence of nuclei I dont see why you can't just H&E the tissue? If you're looking for specific sequences, then maybe molecular biological techniques might be better than histological techniques.

Thanks.

Basically i need to identify any chromosomal mutations and/or abberations, if any, for a disorder or a disease and one of the things that i cant tell the difference between is FISH & paint staining.

All that i have seen/know for fetal nuclei is in the form of FISH staining, because i am having troubles between the two i thought if i could identify differences of one than the other then that could maybe help.

I also need to identify mutations/abberations of disorders/diseases when looking at chromosomes of various staining methods buut focussing on chromosome PAINTS, FISH and traditional karyotying. From this i need to know identify any translocations, deletion, duplications, onversions ant trisomy's etc..

Thanks for your response, please do give your thoughts.

I have found out the the main method for this via FISH although there are various other not so common methods, this is because the process of the other method(s) are slightly more complex than FISH.

Thanks