am staining some cells with antibodies for FACS analysis.
my primary ab conc is 0.2ug/ul.
In one tube i have 1,000,000 cells (the approx volume of these cells is 40ul).i add 1ul of my primary ab to these cells and run my facs
If i have 10,000,000 cells (which is say 100ul of cells). How much of my antibody (ul) should i add to my 10,000,000 cells.
Is it 10ul that i add. Do i need to take into account the volume of my cells?