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| darmic |
 Posted: Tue Mar 25, 2008 2:44 am Post subject: Extraction of Catalase |
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Forum Freshman
Joined: 03 Feb 2008
Posts: 15
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Hi,
Can anyone please tell me how I can extract catalase from a rabbit's liver if it is a possible process?
thanks
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Darren |
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| i_feel_tiredsleepy |
| Posted: Tue Mar 25, 2008 7:07 am Post subject: |
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Forum Senior
Joined: 21 Mar 2008
Posts: 376
Location: Montreal
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I don't know about isolating from animal tissue, there are plenty of procedures for isolating from plants available online. You can buy kits to assay catalase activity though.
Edit: In microbiology labs we just drop peroxide on the bacteria to see if they have catalase or not lol. |
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| CShark |
| Posted: Fri May 09, 2008 11:33 am Post subject: I_feel: RT ? |
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Forum Freshman
Joined: 08 May 2008
Posts: 58
Location: East Coast of Canada
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You must be a med tech, correct ? Where did you go to school ?
Got my R.T from Algonquin |
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| free radical |
| Posted: Fri May 09, 2008 12:09 pm Post subject: Re: Extraction of Catalase |
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Forum Senior
Joined: 24 Sep 2007
Posts: 349
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| darmic wrote: |
Hi,
Can anyone please tell me how I can extract catalase from a rabbit's liver if it is a possible process? |
it should be possible. Ex from pigs:
http://www.jstor.org/pss/3627031
The procedure should be similar. Did you try Google. |
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| i_feel_tiredsleepy |
| Posted: Fri May 09, 2008 12:14 pm Post subject: Re: I_feel: RT ? |
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Forum Senior
Joined: 21 Mar 2008
Posts: 376
Location: Montreal
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| CShark wrote: |
You must be a med tech, correct ? Where did you go to school ?
Got my R.T from Algonquin |
No I'm working on my bachelor's degree in Microbiology and Immunology at McGill University, but I've taken the laboratory course in medical microbiology so I'm familiar with the tests for diagnosis and identification of pathogens. |
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| CShark |
| Posted: Fri May 09, 2008 12:43 pm Post subject: |
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Forum Freshman
Joined: 08 May 2008
Posts: 58
Location: East Coast of Canada
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| I see. The med lab micro course is three years, two theory and labs, and one clinical. |
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| i_feel_tiredsleepy |
| Posted: Fri May 09, 2008 12:48 pm Post subject: |
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Forum Senior
Joined: 21 Mar 2008
Posts: 376
Location: Montreal
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| CShark wrote: |
| I see. The med lab micro course is three years, two theory and labs, and one clinical. |
Ya I've taken two year long lab courses and and a ton of theory, I won't do clinical obviously though. The lab course for me was more designed to become familiar with the techniques for studying viruses, bacteria, and human immune cells. The diagnosis section I think they put in just to fill space lol. I have a parasitology lab next year too. |
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| Kenny Klassen |
| Posted: Fri May 09, 2008 4:11 pm Post subject: |
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Forum Freshman
Joined: 15 Mar 2008
Posts: 65
Location: Quesnel BC
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Out of curosity why are you trying to remove a catlyst from a rabbit liver, is the rabbit alive, do you care if it still will be at the end if so?
_________________
In the study of life in todays socity there are sevral truth we must face the folowing are the ones I have manged to come to terms with:
1. You will probably end up doing something related to Cancer, Aids, or some other dieases that incovinces the genral public
2. In order to make money you either will be doing the afore mentioned research because that is where all the free grant money is, working for a company, or having to teach people some of whom really should not be there
3. You cannot take up reserach biologist as a hobby inless you have another substancial form of income |
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| i_feel_tiredsleepy |
| Posted: Fri May 09, 2008 7:34 pm Post subject: |
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Forum Senior
Joined: 21 Mar 2008
Posts: 376
Location: Montreal
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| Kenny Klassen wrote: |
| Out of curosity why are you trying to remove a catlyst from a rabbit liver, is the rabbit alive, do you care if it still will be at the end if so? |
I'm quite sure the rabbit is long dead by the time you get to it's liver  .
Maybe he needs some catalase and he's got a few extra rabbits lying about. |
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| Kenny Klassen |
| Posted: Fri May 09, 2008 8:47 pm Post subject: |
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Forum Freshman
Joined: 15 Mar 2008
Posts: 65
Location: Quesnel BC
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I rarely keep well stocked on rabits but your proably right
_________________
In the study of life in todays socity there are sevral truth we must face the folowing are the ones I have manged to come to terms with:
1. You will probably end up doing something related to Cancer, Aids, or some other dieases that incovinces the genral public
2. In order to make money you either will be doing the afore mentioned research because that is where all the free grant money is, working for a company, or having to teach people some of whom really should not be there
3. You cannot take up reserach biologist as a hobby inless you have another substancial form of income |
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