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georginho_juventusygr
Posted: Wed Mar 26, 2008 4:21 pm    Post subject: A Stupid Question Reply with quote

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Will do an experiment tomorrow to know how much vitamin C is in a bottle of soft drink.

Will use the internal standard method of chromatography.

It states in the instructions that I have to make a solution which has vitamin C and isonicotinic acid in. Both of them have to have concentration of 10 ppm. This solution is my internal standaard.

So, if I understand well, I have a solution of 10 ppm vitamin C and a solution of 10 ppm isonicotinic acid in a flask. How the heck can I calculate the concentration of the solution?
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organic god
Posted: Wed Mar 26, 2008 6:32 pm    Post subject: Reply with quote

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perhaps if you titrate it against a standard solution you will find its concentration.
probably something like 0.1M NaOH. find the endpoint and work backwards to find the concentration
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SteveF
Posted: Thu Mar 27, 2008 4:32 am    Post subject: Reply with quote

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Use this method for preparing any standard solution.

Start with a solution of known concentration. Perhaps you prepared it yourself from the chemicals on the shelf. Then use successive dilutions to achieve the concentration you want.

 
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